Kinetic studies of the phosphorolysis of polyadenylic acid.
نویسندگان
چکیده
A preparation of polynucleotide phosphorylase from Micrococcus Zysodei&icus that reversibly catalyzes the formation of long chain polynucleotides from nucleoside 5’-diphosphates has been described previously (2). The general features of the reaction appear similar to those reported earlier by GrunbergManago et al. (3, 4), who used preparations from Azotobacter vinelandii. The over-all reaction for ADP can be expressed as:
منابع مشابه
The effect of chain length on the phosphorolysis of oligonucleotides by polynucleotide phosphorylase.
The kinetics of phosphorolysis of compounds in the series (Ap),A (II = 2 to 8), p(Ap),A (n = 2 to 4), and (Up)I;cT, (II = 3 to 6) has been studied. K, and V,,, values were obtained for all compounds. K, decreases with increasing chain length and reaches a minimum value with compounds containing 5 phosphate residues. With the shorter compounds the dependence of K, on the nucleoside residues is a...
متن کاملEquilibrium and Kinetic Studies on the Adsorption of Acid Yellow 36 Dye by Pinecone
Background & Aims of the Study: Dyes have significant role in environmental problems, due to their toxic effects on the food chain and sources of water. The purpose of this research was to study the adsorption of acid yellow 36 dye onto pinecone using batch system. Materials & Methods: This research was performed at laboratory scale and batch system. Equilibrium isotherms...
متن کاملKinetic Studies of the Catalytic Oxidation of Toluene to Benzoic Acid in the Liquid Phase
The catalytic oxidation of toluene to benzoic acid in the liquid phase has been studied in an un-stirred tank reactor. Low concentration of acetic acid was added in order to increase the solubility of the catalyst in toluene. The dependence of the rate of reaction on the concentration of toluene catalyst and oxygen pressure was studied. It has been suggested that liquid phase oxidation of t...
متن کاملInteraction of polyadenylic acid with the mercury electrode.
Interactions of polyadenylic acid with the mercury electrode were followed by means of direct current (d.c.), alternating current (a.c.), and pulse polarography. It was ascertained that the electrode reaction of polyadenylic acid did not differ significantly from the reaction of its monomeric components. In contrary to the latter reaction, the polarographic reduction of poly adenylic acid take...
متن کاملPhysical Chemistry Studies of Acid Dye Removal from Aqueous Media by Mesoporous Nano Composite: Adsorption Isotherm, Kinetic and Thermodynamic Studies
In this research, SBA-15/Polypyrrole (SBA-15/PPy) mesoporous nanocomposite was synthesized, characterized and applied for Acid Blue 62 (AB62) adsorption as a textile dye from aqueous solution. In order to evaluate the structural properties of synthesized adsorbent, FT-IR, FESEM and TEM images, XRD, and BET techniques were applied. Some parameters such as pH, dosage, and time which affect the ba...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- The Journal of biological chemistry
دوره 236 شماره
صفحات -
تاریخ انتشار 1961